A description of the original DNAWorks can be found in our publication (Hoover, D. and Lubkowski, J., 2002)

For further improvements in the performance of DNAWorks, feedback on the success or failure of gene synthesis using DNAWorks is very important. Please let us know about the outcome of your synthesis experiments.

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Job Name: You must enter a name for your job. Acceptable characters are [A-Za-z0-9_].

E-mail Address (optional):

Entering your e-mail address is entirely voluntary. The results will be made available online at the end of the program run, but if an address is entered, the results will also be sent to that address. Online results will be deleted after two days.

Mutant run (optional): All parameters from original logfile will override those from this page, except for job name, email and sequences.

Enable mutant run:

Codon Frequency Table:

GCG CodonFrequency format only. For more organisms, check out the Codon Usage Database
Choice 3 - Upload codon frequency file:

Parameters:

°C to °C    
nt to nt
Random:
 
%
M      
M      
M      
     
     
     

Restriction Site Screen (optional):

Sites selected will be excluded from the protein coding region of the synthetic gene

Custom Site Screen (optional):

Input your own site to be excluded from the protein coding region(s) of the synthetic gene (format)

Weights (optional):

TWT: CWT: RWT: MWT: GWT: AWT: LWT: PWT: FWT:

Sequence(s) (formats):Enter up to 99 sequence elements.

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