This database provides RPKM values for the transcriptome of manually microdissected glomeruli from untreated male Sprague-Dawley rats (200-250 g BW). Please cite: Lee JW, Chou CL, Knepper MA. Deep Sequencing in Microdissected Renal Tubules Identifies Nephron Segment-Specific Transcriptomes. J Am Soc Nephrol. 2015 PMID: 25817355.
Brief Method: Rat renal tubule segments manually dissected under microscope were lysed in cell lysis buffer containing reverse transcriptase and oligo-dT primers. After reverse transcription of poly(A)-tailed mRNA transcripts, cDNAs were amplified and sequenced using Illumina HiSeq 2000 sequencer. FASTQ sequences were mapped to rat reference genome (rn5).
How to search the database: Enter official gene symbol or annotation string in the Search Box at the right upper corner of the table.
|Gene||RefSeq ID||Annotation||G1||G2||G3||G4||G5||median RPKM|