National Heart, Lung, and Blood Institute Epithelial Systems Biology Laboratory

RNA-seq Analysis of Microdissected Rat Glomeruli

This database provides RPKM values for the transcriptome of manually microdissected glomeruli from untreated male Sprague-Dawley rats (200-250 g BW). Please cite: Lee JW, Chou CL, Knepper MA. Deep Sequencing in Microdissected Renal Tubules Identifies Nephron Segment-Specific Transcriptomes. J Am Soc Nephrol. 2015 PMID: 25817355.

Brief Method: Rat renal tubule segments manually dissected under microscope were lysed in cell lysis buffer containing reverse transcriptase and oligo-dT primers. After reverse transcription of poly(A)-tailed mRNA transcripts, cDNAs were amplified and sequenced using Illumina HiSeq 2000 sequencer. FASTQ sequences were mapped to rat reference genome (rn5).

How to search the database: Enter official gene symbol or annotation string in the Search Box at the right upper corner of the table.

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Gene RefSeq ID Annotation G1 G2 G3 G4 G5 median RPKM